Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Growth hormone secretagogue receptor (GHS-R) is expressed in type II but rarely in type III taste cells of circumvallate papillae. ( A – C ) PLCβ2-GFP, green; anti-GHS-R, red; and merged images, respectively. In ( C ), the yellow arrow points to a representative taste cell that expresses PLCβ2 but not GHS-R, and the white arrow highlights a PLCβ2-negative, GHS-R-positive taste cell. ( D – F ) GAD67-GFP, green; anti-GHS-R, red; and merged images, respectively. ( G ) Anti-GHS-R antibody incubated on a representative section of circumvallate papillae from a GHS-R-deficient mouse (negative control). Nuclear staining is shown in blue in all figures.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Incubation, Negative Control, Staining, Immunofluorescence

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: GHS-R is expressed in type II but rarely in type III taste cells of the fungiform papillae. ( A – C ) PLCβ2-GFP, green; anti-GHS-R, red; and merged images, respectively. ( D – F ) GAD67-GFP, green; anti-GHS-R, red; and merged images, respectively.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Immunofluorescence

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Body weight and food intake in wild-type (WT) and GHS-R-deficient mice on a high-fat diet. While not showing a significant difference in body weight ( A ), Ghsr −/− females consumed less compared to WT females ( B ). Alternatively, male Ghsr −/− mice ( C , D ) showed a decrease in body weight by week 5 of the HFD and no significant differences in food intake compared to their WT counterparts.* p -value < 0.05.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques:

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Linoleic acid responsiveness assessed in a conditioned taste aversion assay showed changes in Ghsr −/− female mice. ( A ). WT female mice (n = 15) revealed a significant aversion to LA at 10 µM, similar to that seen in WT males ( n = 15) ( B ). Ghsr −/− females ( n = 11) showed no significant differences in the LiCl compared to the NaCl group across all concentrations of LA ( C ). Male mice lacking GHS-R ( n = 18) showed aversion at 10 µM LA, similar to WT mice ( D ). WT and Ghsr −/− females ( E ) and males ( F ) exhibited similar lick ratios to the control solutions, sucrose (100 mM), and denatonium benzoate (3 mM). * p -value < 0.05, ** p -value ≤ 0.01, and *** p -value < 0.0001.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Control

Journal: Journal of Vascular Research

Article Title: Evaluation of Metabolism-Associated Proteins in Abdominal Aortic Aneurysm

doi: 10.1159/000543768

Figure Lengend Snippet: Representative immunohistochemical detection of FNDC5 ( a , b ), follistatin ( c , d ), activin A ( e , f ), ghrelin ( g , h ), ghrelin receptor ( i , j ), and α-actin ( k ) in non-aneurysmal control aorta ( a , c , e , g , i ) and AAA ( b , d , f , h , j , k ), at ×200 magnification. Scale bar corresponds to 10 µm.

Article Snippet: Sections were incubated with primary antibodies against FNDC5 (diluted 1:200, rabbit polyclonal, #23995-I-AP, Proteintech, UK); follistatin (diluted 1:300, rabbit polyclonal, #PA5-114319, Thermo Fisher Scientific, USA); activin A (diluted 1:50, goat polyclonal, #PA5-47004, Thermo Fisher Scientific, USA); ghrelin (diluted 1:150, mouse monoclonal, #H00051738-M01, Novus Biologicals, UK); ghrelin receptor (diluted 1:150, rabbit polyclonal, #NBP2-16655, Novus Biologicals, UK); and actin α-smooth muscle (diluted 1:5,000, mouse monoclonal, #A5228, Sigma-Aldrich, USA).

Techniques: Immunohistochemical staining, Control

Journal: Journal of Vascular Research

Article Title: Evaluation of Metabolism-Associated Proteins in Abdominal Aortic Aneurysm

doi: 10.1159/000543768

Figure Lengend Snippet: Representative immunohistochemical detection of activin A ( a ), ghrelin receptor ( b ), and α-actin ( c ) in AAA, at ×400 magnification. Scale bar corresponds to 10 µm.

Article Snippet: Sections were incubated with primary antibodies against FNDC5 (diluted 1:200, rabbit polyclonal, #23995-I-AP, Proteintech, UK); follistatin (diluted 1:300, rabbit polyclonal, #PA5-114319, Thermo Fisher Scientific, USA); activin A (diluted 1:50, goat polyclonal, #PA5-47004, Thermo Fisher Scientific, USA); ghrelin (diluted 1:150, mouse monoclonal, #H00051738-M01, Novus Biologicals, UK); ghrelin receptor (diluted 1:150, rabbit polyclonal, #NBP2-16655, Novus Biologicals, UK); and actin α-smooth muscle (diluted 1:5,000, mouse monoclonal, #A5228, Sigma-Aldrich, USA).

Techniques: Immunohistochemical staining

Journal: Royal Society Open Science

Article Title: The unexpected loss of the ‘hunger hormone’ ghrelin in true passerines: a game changer in migration physiology

doi: 10.1098/rsos.242107

Figure Lengend Snippet: Phylogenetic tree showing the loss of ghrelin in Eupasserine birds. The figure illustrates the loss of ghrelin after Acanthisitta split from Eupasserines during the Palaeogene. While the receptor (GHS-R) is present even in Eupasserines, the ligand replacing ghrelin is still unknown. The phylogenetic tree was obtained from TimeTree ( http://timetree.org/ ; last accessed 21 April 2023). The x -axis shows the age of the event in million years ago (Ma). Bird drawings were obtained from the Lynx Edicions’ Handbook of the Birds of the World with permission of Alada Gestió Empresarial S.L.

Article Snippet: The GHS-R concentration of plasma samples collected from two different bird species (the garden warbler and the common quail) was quantified using a commercially available enzyme-immunoassay kit for chicken ghrelin receptor (MyBioSource).

Techniques: